CHEM120 OL, Week 7 Lab

CHEM120

10-16-2020

OL Lab 13: Protein Synthesis

Learning Objectives

 Explain the translation process from mRNA to amino acid

 Explain post-translational modification of proteins

 Explain protein synthesis processing in the ribosome

 Explain the primary, secondary, tertiary and quaternary structures of protein

 Explain the basic principles of mass spectrometry (MALDI-TOF)

Introduction

In the Protein Synthesis lab, you will learn about the difference between protein

synthesis in prokaryote (using E. coli) and eukaryote (using Chinese hamster ovarian

(CHO) cells).

Prepare recombinant Erythropoietin and use the mass spectrometer

Your first task in the lab will be to prepare recombinant erythropoietin (EPO) that is

transfected into E. coli and CHO cells. The lab assistant will prepare the recombinant

EPO and you will measure the mass to charge ratio using a mass spectrometer. Not

sure how to handle the mass spectrometer? No worries! You can just take out your

labpad and find an animated video to learn the basics.

Study the translation process from mRNA to amino acids

You will also learn about the translation process from mRNA to amino acids and how

amino acids are assembled to proteins. A 3D animation will describe how codons are

translated into amino acids, how these amino acids are joined together by peptide

bonds creating a primary structure of protein, and how the primary structure is folded

into secondary, tertiary and quaternary structure.

Investigate doping in bike athletes

In the last part of the Protein Synthesis lab, you will use mass spectrometry to

investigate if any athletes are using recombinant human erythropoietin (rhEPO) as a

doping substance. You will do so by collaborating with the doping agent who collects

urine samples at a large bicycle race.

Will you be able to detect if any of the athletes are using doping?

Part 1: Complete Labster Lab: Protein Synthesis

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