AH09 Unknown Pathogen Analysis

Objective: To identify an unknown pathogen(s) based on the given assays

developed during this course.

Notes:

 Two samples from the hospital was brought to your lab for

diagnosis. After culturing the samples on LB agar plates another coworker began the analysis. However, due to an emergency your coworker has left the remaining work to you. Before leaving your coworker informs you the 2 liquid culture samples were accidentally

combined before the Gram stain was started. However, the 2

cultures remain separated on LB plates for future tests.

o GOAL: To correctly identify the 2 pathogens.

Results:

 Gram (+) coccus clusters

 Gram (-) bacillus rods

 MacConkey plate

o Culture A – no growth

o Culture B – lactose fermentation is present

 Zone 1 – growth is apparent

 Zone 2- colony isolates begin to form.

 Zone 3 – single colony isolates.

 BAPo Culture A- beta-hemolysis

o Culture B- no beta-hemolysis

 EMBo Culture A- No growth

o Culture B- colonies with green metallic sheen

 Catalase Testo Culture A- positive

o Culture B- positive

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